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MagPure FFPE DNA/RNA Kit

FFPE DNA/RNA Early Partition Magnetic Workflow Note

Cat. No. IVD3026 · Representative magnetic workflow for parallel purification of DNA and RNA from the same FFPE input

Shared pretreatment Magnetic purification Partition / Elution

Workflow — Shared Pretreatment and Dual Magnetic Purification Paths

DNA / RNA Partition

After FFPE lysis and centrifugation, the RNA-containing supernatant and DNA-containing pellet are processed separately. This early partitioning step distinguishes the workflow from sequential adsorption methods where DNA and RNA are separated during magnetic binding.

RNA Path

≈ 1 h 4 min

DNA Path

≈ 2 h 40 min

Estimated Total Time≈ 4.7 h

How to read this note

1. Workflow structure
This workflow separates shared FFPE pretreatment from two downstream magnetic bead purification paths. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. Both downstream paths follow magnetic bead-based purification after internal partitioning of the lysate.

2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. For short protocol ranges, the timeline uses the midpoint. The total time reflects the shared pretreatment stage plus both downstream purification paths under the standard protocol, since both DNA and RNA are recovered from the same FFPE input.

3. Workflow characteristics
This workflow separates DNA and RNA before the magnetic binding stage. RNA is recovered from the supernatant after an 80°C incubation, while the DNA-containing pellet is taken through a second lysis and a 56°C / 90°C sequence before magnetic binding. The downstream purification logic is magnetic rather than column-based, with bead binding, magnetic separation, wash, drying and elution steps in each path.

4. Practical considerations
The most important handling point is the partition step after centrifugation. Supernatant transfer should preserve the DNA-containing pellet for the second path, while the RNA path should proceed without unnecessary delay. The RNA path is shorter but includes a longer 80°C incubation than the column split workflow, whereas the DNA path remains the dominant contributor to total time because it includes a second digestion and crosslink-reversal stage before magnetic purification.